light microscope Search Results


stellaris 5 cryo confocal laser scanning microscope  (Danaher Inc)
94
Danaher Inc stellaris 5 cryo confocal laser scanning microscope
a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica <t>Stellaris</t> <t>5</t> cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.
Stellaris 5 Cryo Confocal Laser Scanning Microscope, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stellaris 5 cryo confocal laser scanning microscope/product/Danaher Inc
Average 94 stars, based on 1 article reviews
stellaris 5 cryo confocal laser scanning microscope - by Bioz Stars, 2026-03
94/100 stars
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olympus bx43 light microscope  (Evident Corporation)
99
Evident Corporation olympus bx43 light microscope
a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica <t>Stellaris</t> <t>5</t> cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.
Olympus Bx43 Light Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
olympus bx43 light microscope - by Bioz Stars, 2026-03
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film thicknesses  (Nikon)
94
Nikon film thicknesses
a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica <t>Stellaris</t> <t>5</t> cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.
Film Thicknesses, supplied by Nikon, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/film thicknesses/product/Nikon
Average 94 stars, based on 1 article reviews
film thicknesses - by Bioz Stars, 2026-03
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bx 53 darkfield microscope  (Evident Corporation)
99
Evident Corporation bx 53 darkfield microscope
a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica <t>Stellaris</t> <t>5</t> cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.
Bx 53 Darkfield Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bx 53 darkfield microscope/product/Evident Corporation
Average 99 stars, based on 1 article reviews
bx 53 darkfield microscope - by Bioz Stars, 2026-03
99/100 stars
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transmitted light microscope  (Carl Zeiss)
97
Carl Zeiss transmitted light microscope
a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica <t>Stellaris</t> <t>5</t> cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.
Transmitted Light Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transmitted light microscope/product/Carl Zeiss
Average 97 stars, based on 1 article reviews
transmitted light microscope - by Bioz Stars, 2026-03
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ix73 inverted epifluorescence microscope  (Evident Corporation)
99
Evident Corporation ix73 inverted epifluorescence microscope
a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica <t>Stellaris</t> <t>5</t> cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.
Ix73 Inverted Epifluorescence Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ix73 inverted epifluorescence microscope/product/Evident Corporation
Average 99 stars, based on 1 article reviews
ix73 inverted epifluorescence microscope - by Bioz Stars, 2026-03
99/100 stars
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inverted light microscope  (Carl Zeiss)
97
Carl Zeiss inverted light microscope
a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica <t>Stellaris</t> <t>5</t> cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.
Inverted Light Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inverted light microscope/product/Carl Zeiss
Average 97 stars, based on 1 article reviews
inverted light microscope - by Bioz Stars, 2026-03
97/100 stars
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bx43 transmitted light microscope  (Evident Corporation)
99
Evident Corporation bx43 transmitted light microscope
a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica <t>Stellaris</t> <t>5</t> cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.
Bx43 Transmitted Light Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bx43 transmitted light microscope/product/Evident Corporation
Average 99 stars, based on 1 article reviews
bx43 transmitted light microscope - by Bioz Stars, 2026-03
99/100 stars
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light sheet microscope  (Evident Corporation)
94
Evident Corporation light sheet microscope
a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica <t>Stellaris</t> <t>5</t> cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.
Light Sheet Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/light sheet microscope/product/Evident Corporation
Average 94 stars, based on 1 article reviews
light sheet microscope - by Bioz Stars, 2026-03
94/100 stars
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szx7 stereomicroscope  (Evident Corporation)
97
Evident Corporation szx7 stereomicroscope
a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica <t>Stellaris</t> <t>5</t> cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.
Szx7 Stereomicroscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/szx7 stereomicroscope/product/Evident Corporation
Average 97 stars, based on 1 article reviews
szx7 stereomicroscope - by Bioz Stars, 2026-03
97/100 stars
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microscope zeiss axio observer d1 400x  (Carl Zeiss)
99
Carl Zeiss microscope zeiss axio observer d1 400x
a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica <t>Stellaris</t> <t>5</t> cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.
Microscope Zeiss Axio Observer D1 400x, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microscope zeiss axio observer d1 400x/product/Carl Zeiss
Average 99 stars, based on 1 article reviews
microscope zeiss axio observer d1 400x - by Bioz Stars, 2026-03
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primo star hal led microscope  (Carl Zeiss)
90
Carl Zeiss primo star hal led microscope
a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica <t>Stellaris</t> <t>5</t> cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.
Primo Star Hal Led Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica Stellaris 5 cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.

Journal: bioRxiv

Article Title: Neutrophils secrete exosome-associated DNA to resolve sterile acute inflammation

doi: 10.1101/2024.04.21.590456

Figure Lengend Snippet: a. An overlay image of PMNs on fibrinogen-fibronectin coated Quantifoil grids stimulated with 100 nM LTB 4 for 15 min in the presence of SYTOXgreen, plunge-frozen, and imaged on Leica Stellaris 5 cryo-confocal, showing the reflected light (gray) and extracellular DNA (green). Dashed yellow lines highlight PMNs on the grid square. The scale bar is 50 μm. b. A low-magnification TEM image (6500x) of the highlighted region (red box) in panel a, is overlaid with the SYTOX green fluorescence signal. The cyan box indicates the area where cryo-ET data was acquired. The scale bar is 500 nm. c. A slice through the tomogram of the region highlighted (cyan box) in panel b, with insets showing ‘bead-on-string’ like structures (yellow arrowheads). The scale bar is 100 nm. d. Segmentation of vesicles (<100 nm in orange, >100 nm in olive green) and the DNA (cyan) corresponding to the tomogram in panel c. The scale bar is 100 nm. See associated Movie 1. e. Representative western blot of exosomes obtained from fractions 4-9 of 5-40% density gradient centrifugation from PMNs stimulated with 100 nM LTB 4 and treated with 50 µg/ml DNase I followed by immunoblotting for FLAP, 5LO, and histone H3. N=3. f. Bar graph showing the quantifications of the band intensity of FLAP, 5LO, and histone H3, in control and DNase I treated exosome samples. Data points (red dots) from 3 independent experiments were plotted as mean ± s.e.m. P values determined using two-way ANOVA are reported. g. Schematic illustration of DNA packaging and secretion during the nuclear budding and the fusion of NE-MVB with the PM. Note the change in the orientation of the LBR N-terminal Tudor domain as it moves from the inner nuclear membrane to the PM.

Article Snippet: The quality of cells and grids was assessed in widefield mode on a Stellaris-5 cryo-confocal laser scanning microscope equipped with a cryo-stage and x50/0.9 numerical aperture objective (Leica Microsystems).

Techniques: Fluorescence, Tomography, Western Blot, Gradient Centrifugation, Membrane